White Stork (Ciconia ciconia) nesting colony by the side of the road at Rasmalo in the Algarve.

Plus at least one in black plumage,

Tags: WhiteStork

Ballast Toadflax (Linaria spartea)





Pink Pirouette (Silene colorata)













White Spanish Broom (Cytisus multiflorus)



Waxbill (Estrilda astrild)

Corn Bunting (Emberiza calandra)



Snipe (Gallinago gallinago)





Common Sandpiper Actitis hypoleucus)







Spoonbill (Platalea leucorodia)



Chichchaff (phylloscopus colybita)



Cattle Egret (Bubulcus ibis)

























Black-winged Stilt (Himantopus himantopus)













Phlomis purpurea







Cistus sp





Smilax aspera









Lichen Sp

















Yellow Bee Orchid (Ophrys lutea)





Mirror Orchid (Ophrys speculum)















BumbleBee Orchid (Ophrys bombyliflora)



White Stork (Ciconoa ciconia)

Tags: CornBunting, CommonSandpiper, CattleEgret, Chiffchaff, Spoonbill, Snipe, WhiteStork, JerusalemSage, MirrorOrchid, Landscape, BumbleBeeOrchid, YellowBeeOrchid, Smilax

Basket Stinkhorn (Clathrus ruber)

Tags: BasketStinkhorn

Lichen Sp?





Snail Sp?



















Bee Sp?





Tick Sp?

Tags: LichenSp, SnailSp, BeeSp, TickSp

On the road down to the sea

Lichen Sp





Pellia Sp







Fossombronia Sp







Fissidens Sp





Mediterranean Nettle (Urtica membranacea)



Green Winged Orchid (Orchis morio)







Lesser Snapdragon (Misopates orontium)





Cage Fungus (Clathrus ruber)



Ant Sp



Barbary Nut (Gynandris sisyrinchium / Moraea sisyrinchium)

Tags: LichenSp, BryophyteSp, GreednWingedOrchid, CageFungus, AntSp, BarbaryNut, MediterraneanNettle, LesserSnapdragon

Red Kite (Milvus milvus)

Tags: RedKite

Brent (Branta bernicla)







Lecanora sulphurea?





Pyrrosphora quercina

C+ orange (right)
KC+ orange (left)
UV+ orange





?



C+ Rose





Dirina massillienis f sorrediata

The Norfolk Fungus Study Group

DNA team

at work at

Wheatfen

under the watchful gaze of

Wheatfen's founder

Ted Ellis



Working surfaces are cleaned and sterilised with 10% thin domestic bleach in tap water with a drop of washing up liquid.
This is vital to prevent contamination, particularly if there of risk of amplified DNA on the surfaces.



A variety of pipettes, epindorf tubes & gloves needed for the process



Preparing numbered 1.5ml epindorf tubes with 100µl of extraction buffer ready for the samples.



Fungal specimens submitted in labelled packets







Using sterilised tweezers, razor blades and needles, and sterilising instruments in bleach between specimens,
small samples of fungal tissue are cut or torn off from the samples for testing.

Each sample is then placed in an appropriately numbered 1.5ml epindorf tube containing extraction buffer as mentioned above.



The samples are then ground with a sterile (bleached pestle) until the tissue is mostly pulverised.



Once ground teach tube has a further 400µl of extraction buffer added to it.

A seperate set of similarly numbered tubes have 1000µl of wash Buffer added to them.



Preparation of the 5x HOT FIREPol Master MIx

152µl of PCR Grade Water
40µl 5x HOT FIREPol Mix
4µl of prepared ITS1F primer at working concentration
4µl of prepared ITS4 primer at working concentration

Numbered tubes are then prepared, each containing 20µl of this solution





This is the moment when it all comes together and DNA is extracted.

1 A dipstick is dipped into the epindorf tube containing the sample with the extraction buffer three times.
2 The dipstick is then dipped into the tube containing the wash buffer three times
3 The dipstick is then dipped into the PCR mix tube three times to release the DNA



The PCR machine where the prepared samples complete with primers and FIREPol Mastermix are put through a thermal cycle

to amplify the DNA present.



Lab book recording



We've done it



Preparation for GEL electrophoresis

Stecherrinum oreophilum possibly

Tags: FungusSp

Adder (Viperus berus)

Tags: Landscape, Adder

Bristly Haircap Moss (Polytrichum piliforum)







Hoary Fringe-moss (Racomitrium canescens)



Cladonia furcata





Cladonia Sp?





Mossy Stonecrop (Crassula tillaea)







Peltigera Sp?







Heath Plait-moss (Hypnum jutlandicum)





The small white dots in this image are actually a tiny Lichen Coenogonium pinetti



Forked Veilwort (Metzgeria furcata)



Lichen Sp?



Ramalina farinacea?







Rustwort (Nowellia curvifolia)









Bristly Haircap Moss (Polytrichum piliferum) ♂︎plants







Chaenotheca ferruginea



Lateral Cryphaea (Cryphaea heteromalla)



Swan's neck Thyme Moss (Mnium hornum)







Cape Thread-moss (Orthodontium lineare)







Mueller's Pouchwort (Calypogeia muelleriana)

Shallow notches in the underleaves.





A lichen also present Diarthonis spadicea



Minute Pouncewort (Myriocoleopsis minutissima)









Creeping Fingerwort (Lepidozia reptans)



Lichen Sp?











Silesian Feather-moss (Herzogiella selgeri)





Arthonia atra







Lichen Sp?



Capsule without hairs from Ulotta crispa group



Frizzled Pincushion (Plenogemma phyllantha)



Ciliated Fringewort (Ptilidium ciliare)



Dilated Scalewort (Frullenia dilatata)





Two-horned Pincerwort (Cephalozia bicuspidata)



Knieff's Feather-moss (Leptodictyum riparium)



Forcipated Pincerwort (Cephalozia connivens)

Tags: BristlyHaircapMoss, HoaryFringe-moss, Cladoniafurcata, CladoniaSp, ForcipatedPincerwort, Knieff'sFeather-moss, CiliatedFringewort, Coenogoniumpinetti, MossyStonecrop, ForkedVeilwort, BristlyHaircap, Chaenothecaferruginea, LateralCryphaea, Swan's-neckThyme-moss, CapeThread-moss, Mueller'sPouchwort, Diarthornisspadicea, CreepingFingerwort, SilesianFeather-moss, Arthoniaarta, UlottaSp, FrizzledPincushion, Rustwort, LichenSp, PeltigeraSp

Catillaria chalybeia? or Amandinea punctata?

Can only be spearted microscopically





Verrucaria macrostoma f macrostoma with some Myriolecis crenulata



Verrucaria macrostoma





Catillaria chalybeia? or Amandinea punctata?





Amongst other things is this a lichenicolous fungi on Myriolecis albescens



Amongst other things is this a lichenicolous fungi on Myriolecis albescens



Caloplaca (Flavoplaca) ruderum



Caloplaca (Flavoplaca) ruderum with Caloplaca (Variospora) flavescens





Bagliettoa Sp?

Probably Bagliettoa parmigera





Diplotmma alboatrum & Myriolecis albescens





Catillaria chalybeia? or Amandinea punctata?



Grazed Tephromela Atra var atra



Probably Scoliciosporum umbrinum



Probably Scoliciosporum umbrinum



Probably Scoliciosporum umbrinum



Verrucaria macrostoma f macrostoma





Grazed Heamatomma ochroleuchum var?



Tephromela Atra var atra



On the back wet wall. Lepraria Sp? Leprarai incana probably





Dirina masilliensis var?



The Crenulate margins seem less crowded than Lecanora crenulata and the apothecia are brown rather than grey although there is a grey patch in the lower left of of the image

Not Lecanora horiza because of the crenulated edges?

Lecanors Sp?

from Rob

Myriolecis crenulata & M albescens



Probably Amandinea punctata



?







Caloplaca ruderum?





Opegrapha areniseda?

Not sure





Circinnaria calcarea





?

Not sure





Myriolecis crenulata



Diplotomma alboatrum?

Thanks to Norfolk Lichen Recorder for all the help.

From Stewart

Trichoderma gelatinosa growing on the old stromata of Hypoxylon fuscum.

The white fungus is probably the white anamorph of the Trichoderna







Alder Goblet (Ciboria caucus)

No gills





Capillary Thread-moss (Bryum capillare) capsules







from Anne

The tiny cup on the wood in the "mushroom pile turned out to be (I think) Peziza micropus, although some of the spores were wider than the reference Spooner Key B&K 1





Great Scented Liverwort (Conocephelum conicum)



Glistening Inkcap (Coprinellus micaceus)

Hairy stype





Dewdrop Bonnet (Hemimycena tortuosa)



A new fresh fungus.





Spring Hazelcup (Encoelia fufuracea)







Script Lichen Sp (Graphis Sp)?



Lichen Sp



As yet unidentified.

New for the world



Scarlet Bonnet (Atheniella adonis)











Midnight Disco (Pachyella violaceonigra)



From Stewart

Sycamore Felt Gall on a Sycamore (Acer pseudoplatanus) leaf caused by the mite Aceria pseudoplatani



Dialonectria Sp? on ?

From Stewart

Dialonectria episphaeria on Nemania serpens








2.5x life size



200x

From Stewart

I'm glad to say that with a little extra magnification, the yellow blobs that I found on the mossy log were immediately recognisable as Bactridium flavum.  
With 35 records on the mycota, It was something that Reg Evans often found, but it hasn't been seen since 1998.







4x life size

Small discos on the end of a cut trunk.

from Stewart

I had a translucent orange one with a short hairy fringe, which I think came off the end of the log on which Mike found the Dialonectria.
This was Cistella dentata.





Pellia endivifolia





Peniophora incarnata









Fungus Sp? on Fungus Sp?

from Stewart

Calcarisporium arbuscula on Rosellinia britannica





Small disco found on Poplar (Populus Sp) Buds

Calycina gemmarum







Fungus Sp

from Yvonne

Psathyrella bipellis





Old cocoons of a Puss Moth. Created by the caterpillar chewing bark.



Ichneumon sp?









Echinosphaeria canescens



Scarlet Elfcup (Sarcoscypha Sp)



Silver Leaf (Chondostereum purpureum)



Slime Mold Sp

Push Pin Slime Mold (Hemitrichia calyculata)





Slime Mold Sp with accompanying fungus Sp

from Stewart

Polycephalomyces tomentosus on the slime mould Trichia varia









Smokey Bracket 9(Bjerkandera adusta)





Velvet Shank (Flammulina velutipes)





A very young Winter Brownie (Meottomyces dissimulans)





Hazel Woodwart (Hypoxylon fuscum)





White Tubelet (Henningsomyces candidus)







From Anne

The pleurotoid fan shaped fungus turned out to be Crepidotus mollis (the cap peeled I realised after looking at it under the microscope!) FN1 Kibby Vol 4







Trichia varia



Snowy Disco (Lachnum virgineum)



Grey Disco (Mollisia cinerea)

Tags: Landscape, AlderGoblet, CapillaryThread-moss, Pezizamicropus, GreatScentedLiverwort, GlisteningInkcap, DewdropBonnet, SpringHazelcuo, ScriptLichenSp, ScarletBonnet, MidnightDisco, SycamoreFeltGall, Bactridiumflavum, Cistelladentata, Pelliaendivifolia, Peniophoraincarnata, Calcarisporiumarbuscula, Roseliniabritannica, Psathyrellabipeliis, PussMoth, SawflySp, Echinospheariacanescens, SilverLeaf, Hemitrichiacalyculata, Polycephalomycestomentosus, Trichiavaria, SmokeyBracket, VelvetShank, WinterBrownie, Hazelwoodwart, WhiteTubelet, Crepidotusmollis, Trichiavaria, SnowyDisco, GreyDisco, LichenSp

Fungus Sp























Buellia athelia







Arthonia calcarea













Porpidia tuberculosa









Bagleittoa Sp?






Lecanora horiza?











Revolute Beard-moss (Pseudocrossidium revolutum)

Three Suffolk Fens

Thelnetham New Fen, Thelnetham Fen & Hinderclay Fen



Ash (Fraxinus excelsior)













Fungus Sp



Lichen Sp

Lecidella elaeochroma?







Intermediate Screw-moss (Syntrichia montana/intermedia)





Water Screw Moss (Syntrichia latifolia) from the middle of the road.



Snowy Disco (Lachnum virgineum)



Lichen Sp

Xanthoria candelaria or Xanthoria ucrainica?





Bluish Veilwort (Metzgeria fruticulosa)





Elegant Bristle-moss (Orthotrichum pulchellum)



x200









2.5x Life size



x 100



x 100



X400



In a dryer state showing how the leaves curl up.

Neckera smithii form the BBS

Prince of Wales Feather-moss (Neckera smithii)

Confirmed and will be submitted
as
a
new
Vice County record





Drumsticks (Aulocomnium androgynum)


Marble Screw-moss (Syntrichia papillosa_

Tags: Landscape, Waterscrew-moss, FungusSp, LichenSp, ElegantBristle-moss, Drumsticks, PrinceofWalesFeather-moss

White -tipped Bristle-moss (Orthotrichum diaphanum) & Physcia adscendens





Bagliettoa parmigera, Caloplaca sp, Myriolecis albescens & Verrucaria nigrescens f. nigrescens



Bagliettoa parmigera, Caloplaca sp, Myriolecis albescens & Verrucaria nigrescens f. nigrescens



Bagliettoa parmigera



Myriolecis albescens grazed



Myriolecis albescens grazed











Lecanora campestris



Bagliettoa parmigera





Dirina massiliensis





Diplotomma alboatrum









Dirina massiliensis





Sp?



Tephromela atra





Myriolecis crenulata?



Arthonia calcarea







Variospora flavescens







Arthonia calcarea





Variospora flavescens





Tephromela atra



Scolicioscorpon umbrinum?



Variospora flavescens, Veruccaria nigrescnes f. nigrescens & Calopplaca aurella







Myriolecis albescens, Variospora flavescen, Verrucaria nigrescnes f. nigrescens & Diplotoma alboatrum



White-tipped Bristle-moss (Orthotrichum diaphanum) & Anomalous Bristle-moss (Orthotrichum anomalum) plus little bit of


White-tipped Bristle-moss (Orthotrichum diaphanum) & Physcia adscendens

Tags: LichenSp, Landscape